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michelle-case
Team TFS
Team TFS
Here, we demonstrate the benefits of using monolithic columns for the purposes of MS detection and characterization of cell lysate samples containing hundreds proteins. First, we show the effect of eluent flow rate on protein peak resolution and analysis time when using a constant gradient time. Second, the monolith columns are used to analyze molecular weight fractions derived from cell lysates of a mammalian cell line. Application of the methods shown here can be used to optimize the user’s workflow in order to maximize the number of proteins identified in a given sample set while minimizing the total time necessary for high throughput applications.
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Last update:
‎10-20-2014 02:41 PM
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