on 06-17-201603:10 AM - edited on 10-15-202106:59 AM by AnalyteGuru
David M Horn,1 Torsten Ueckert,2 Kai Fritzemeier,2 Katja Tham,2 Carmen Paschke,2 Frank Berg,2 Hans Pfaff,2 Xiaoyue Jiang,1 Shijun Li,1 and Dani Lopez-Ferrer
A new label-free quantification method based on the Minora algorithm is presented and compared to pre-existing label free quantification methods in the Thermo Scientific™ Proteome Discoverer™ software framework. The results of the new algorithm were significantly more accurate across a wide dynamic range compared to spectral counting and “Top N” quantification. The new algorithm was also run on a subset of the Akhilesh Pandey human proteome dataset to identify proteins specific to specific tissue types.
1Thermo Fisher Scientific, San Jose, CA, USA
2Thermo Fisher Scientific, Bremen, Germany