Native Top-Down Analysis of Intact Antibodies using Multiple Dissociation Techniques on a Tribrid Quadrupole Orbitrap Linear Ion Trap Mass Spectrometer

[Orbitrap_SciLib]

Eugen Damoc1, Kristina Srzentić2, Romain Huguet3, Graeme McAlister3, Christopher Mullen3, Philip M Remes3, Jesse D Canterbury3, Mike Senko3, and Vlad Zabrouskov3
ASMS 2019 Poster
Purpose: Evaluate the performance of the new Thermo Scientific™ Orbitrap Eclipse™ Tribrid™ mass spectrometer for native top-down analysis of intact antibodies Methods: Native top-down analysis using High Mass Range MSn (HMRn) operation mode and multiple dissociation methods available on the new Orbitrap Eclipse Tribrid mass spectrometer Results: Demonstrated excellent performance of the new Orbitrap Eclipse Tribrid mass spectrometer for top-down structural characterization of intact antibodies


1Thermo Fisher Scientific, Bremen, Germany 2Thermo Fisher Scientific, Cambridge, Massachusetts 3Thermo Fisher Scientific, San Jose, California