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Multiplexing SIMs on a Novel Bench Top Orbitrap with a Quadrupole Mass Filter to Increase Sensitivity for Peptide Quantitation

Reputable Mentor II
Reputable Mentor II
Maljers L, Cook KL.
ASMS 2011 Poster Note M603
• The Q Exactive high-performance benchtop quadrupole-Orbitrap mass spectrometer is a sensitive quantitation instrument that displays equal or better LOQs than the LOQs reported in the literature for high-end triple quadrupole instruments. The Q Exactive mass spectrometer was found to be the instrument of choice for high-sensitivity quantitation with the following LOQs: o Insulin to an LOQ of 250 pg.mL-1 o Exendin-4 to an LOQ of 5 ng.mL-1 o GLP-1 to an LOQ of 0.1 ng.mL-1 • The use of multiplexed SIMs is very effective for the quantitation of larger peptides such as insulin, exendin-4 and GLP-1. This technology makes the Q Exactive mass spectrometer highly selective. • Because the instrument’s multiplexing capability is coupled with advanced signal processing, its scan speed is fast enough to capture data across narrow UHPLC peaks. For example, 12 - 15 scans were recorded across the 6 s-wide exendin-4 chromatographic peak. • These experimental results portend the applicability of the Q Exactive mass spectrometer for the quantitation of peptides, small proteins and oligonucleotides.

Thermo Fisher Scientific, San Jose, California, USA
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Last update:
‎11-09-2021 01:18 AM
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