on 07-09-201310:33 AM - edited on 10-15-202104:47 AM by AnalyteGuru
Egertson JD, Kuehn A, Merrihew GE, Bateman NW, Maclean BX, Ting YS, Canterbury JD, Marsh DM, Kellmann M, Zabrouskov V, Wu CC, Maccoss MJ. Nat Methods. 2013 Jun 23. In mass spectrometry-based proteomics, data-independent acquisition (DIA) strategies can acquire a single data set useful for both identification and quantification of detectable peptides in a complex mixture. However, DIA data are noisy owing to a typical five- to tenfold reduction in precursor selectivity compared to data obtained with data-dependent acquisition or selected reaction monitoring. We demonstrate a multiplexing strategy, MSX, for DIA analysis that increases precursor selectivity fivefold.