on 07-31-201309:43 AM - edited on 10-15-202111:27 AM by AnalyteGuru
Cunniff J, Wang K, Eiserberg G, Stratton T, Comstock K ASMS 2013 Poster Note Purpose: Demonstrate a workflow for metabolite detection and structure elucidation by HRAM fragmentation-directed secondary analysis using multiple fragmentation techniques.
Methods: Survey analysis of metabolism samples was performed by HRAM full scan and HCD MS2 analysis on a Thermo Scientific™ Q Exactive™ hybrid quadrupole- Orbitrap mass spectrometry. These analysis were processed using fragment ion searching (FISh) to detect structurally related components in the samples for acquistion of MSn data on a Thermo Scientific™ Velos Pro™, dual-pressure linear ion trap mass spectrometer utilizing a combination of HCD and CID fragmentation techniques.
Results: Rapid Metabolite identification and structure elucidation