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Multiple Fragmentation Methods for Small Molecule Characterization on a Dual Pressure Linear Ion Trap Orbitrap Hybrid Mass Spectrometer

Reputable Mentor II
Reputable Mentor II
Comstock K, Huang Y.
Application Note 540
A key advantage of the linear ion trap (LIT) mass spectrometer is its ability to perform multiple stage MS/MS (or MSn) fragmentation on a single precursor and its product ions to yield extensive amounts of structural information and the linkage between them. Coupling a Thermo Scientific Orbitrap mass analyzer to the back of an LIT not only enables parallel data acquisition with high mass accuracy and resolution, but also provides opportunities for post-LIT ion manipulations. Higher-Energy Collisional Dissociation (HCD) was introduced on the Thermo Scientific Orbitrap Velos Pro mass spectrometer as an alternative dissociation method (Figure 1). HCD MS/MS has been demonstrated to display fragment ions resulting from multiple steps of activation.1 It can also determine low m/z product ions. Having access to both dissociation methods can be a significant advantage for small molecule structural elucidation. The two dissociation methods can provide different energy pathways to access fragmentation fingerprints due to the intrinsic mechanistic difference of ion dissociation. Combined with ultra-high resolution and accurate mass, such a platform can offer comprehensive information for confident small molecule structure characterization.

Thermo Fisher Scientific
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Last update:
‎10-15-2021 12:01 PM
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