Peptide mapping is one of the routine methods for biotherapeutics characterization. This technique, combined with mass spectrometry, is utilized in research environments for the determination of the primary sequence of an antibody or the identification of post-translational modifications. UV is often used as a unique detection tool, for instance in stability studies. The data interpretation of these experiments is based on retention times as qualitative and peak area as quantitative information. The high complexity of the sample emphasizes the need for a highly efficient separation that can be achieved with the Thermo Scientific ™ Acclaim ™ column technolology. 1 In addition, for a reliable comparison of different samples, for instance of a reference standard to a new batch of antibody, high retention time reproducibility is mandatory for reliable peptide identification and correct data interpretation.