Monoclonal Antibody Analysis on a Reversed-Phase C4 Polymer Monolith Column

[AnalyteGuru_KB3]

To demonstrate the separation of a monoclonal antibody (mAb) and an
antigen-binding fragment (Fab) from variants or degradation products,
on a reversed-phase C4 (butyl) monolith column using UV detection. The separation is conducted on a 500 µm x 10 cm Thermo Scientific™ ProSwift™ C4 RP-5H column using conventional water/acetonitrile based eluents.