Modifying the Ion Optics and Scan Sequences on a Tribrid MS to Improve Sensitivity, Duty Cycle, and Overall Instrument Ease-of-Use

[Orbitrap_SciLib]

Graeme C. McAlister1, Mike Goodwin1, Raman Mathur1, Lee Earley1, Oliver Lange2, Romain Huguet1, Vlad Zabrouskov1, and Mike Senko1.
ASMS 2019 Poster
Purpose: We modified the ion optics on a Tribrid mass spectrometer to improve its quadrupole selectivity, its high-mass range transmission, and its overall FTMSn duty cycle. Methods: We assessed these ion optics and scan cycle changes using a standard calibration solution, an intact protein standard, and an LC-MS analysis of a complex cellular lysate. Results: All together these ion optics modifications improve the sensitivity and selectivity of the mass spectrometer while increasing its effective analytical mass range.


(1) Thermo Fisher Scientific, San Jose, CA (2) Thermo Fisher Scientific, Bremen, Germany