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Reputable Mentor II
Reputable Mentor II

Edward T. Chouchani1,2*, Lawrence Kazak1,2*, Mark P. Jedrychowski2, Gina Z. Lu1,2, Brian K. Erickson2, John Szpyt2, Kerry A. Pierce3, Dina Laznik-Bogoslavski1, Ramalingam Vetrivelan4, Clary B. Clish3, Alan J. Robinson5, Steve P. Gygi2 & Bruce M. Spiegelman1,2

Brown and beige adipose tissues can dissipate chemical energy as heat through thermogenic respiration, which requires uncoupling protein 1 (UCP1)1,2. Thermogenesis from these adipocytes can combat obesity and diabetes3, encouraging investigation of factors that control UCP1-dependent respiration in vivo. Here we show that acutely activated thermogenesis in brown adipose tissue is defined by a substantial increase in levels of mitochondrial reactive oxygen species (ROS). Remarkably, this process supports in vivo thermogenesis, as pharmacological depletion of mitochondrial ROS results in hypothermia upon cold exposure, and inhibits UCP1-dependent increases in whole-body energy expenditure. We further establish that thermogenic ROS alter the redox status of cysteine thiols in brown adipose tissue to drive increased respiration, and that Cys253 of UCP1 is a key target. UCP1 Cys253 is sulfenylated during thermogenesis, while mutation of this site desensitizes the purine-nucleotide-inhibited state of the carrier to adrenergic activation and uncoupling. These studies identify mitochondrial ROS induction in brown adipose tissue as a mechanism that supports UCP1-dependent thermogenesis and whole-body energy expenditure, which opens the way to improved therapeutic strategies for combating metabolic disorders.
1. Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA. 2. Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA. 3. Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA. 4. Department of Neurology, Harvard Medical School, Boston, Massachusetts 02215, USA. 5MRC Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, UK.

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‎10-15-2021 10:56 AM
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