on 04-29-201410:00 AM - edited on 10-15-202107:24 AM by AnalyteGuru
Wang J, Christison TT, Misuno K, Lopez L, Huhmer AF, Huang Y, Hu S. Anal Chem. 2014 Apr 26. A highly sensitive platform coupling capillary ion chromatography (CapIC) with Q Exactive mass spectrometer has been developed for metabolic profiling of head and neck squamous cell carcinoma (HNSCC) cells. The CapIC has demonstrated outstanding separation and peak shape for anionic polar metabolites and the sensitivities increased by up to 100-fold compared to reversed-phase liquid chromatography and hydrophilic interaction chromatography running at either high- or micro-flow rates. The detection limits for a panel of standard metabolites were between 0.05 to 0.5 nmol/L (0.2 and 3.4 fmol) at signal-to-noise ratio of 3. The intra-day (n = 6) relative standard deviations (RSD) of retention time and intensity were below 8%. This platform was applied to an untargeted metabolomic analysis of head and neck cancer cells and stem-like cancer cells. Differential metabolomics analysis identified significant changes in energy metabolism pathways, e.g., glycolysis and tricarboxylic acid cycle. These experiments demonstrate CapIC/MS as a powerful metabolomics tool by providing enhanced resolution and sensitivity of polar metabolites combined with the high resolution and accurate mass measurement (HR/AM) capabilities to differentiate isobaric metabolites.