Anastasia Kalli1, Bryson Bennett2, Junhua Wang1, Caroline Ding1 and Ralf Tautenhahn1
Utilize ultra-high resolution mass spectrometry for resolving 13C and 15N double-labeled metabolites extracted from MDB-MA-231 cells
Perform metabolite ID with Thermo Scientific™ Compound Discoverer™ 2.0 software in an automated manner through the ChemSpider search node, a local metabolite compound database based on accurate mass measurements and mzCloud HRAM fragmentation library
Chromatographic separation was performed with, either, reverse-phase ion-pairing chromatography, or, hydrophilic interaction chromatography (HILIC)
Mass spectrometric analysis was performed on a Thermo Scientific™ Q Exactive™ Plus MS and on a Thermo Scientific™ Q Exactive™ HF Orbitrap mass spectrometer operated at a resolution of 140,000 and 240,000, respectively
The ultra-high resolution provided by the Q Exactive Orbitrap systems allowed for confident and accurate analysis of 13C and 15N double-labeled metabolites extracted from MDB-MA-231 cells
1Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, CA 95134, USA
2Calico Labs, South San Francisco, CA 94080, USA
United States
