Metabolome identification using LC-MSn Orbitrap-based mass spectrometry

[Orbitrap_SciLib]

David Peake, Ioanna Ntai, Amanda Souza and Ralf Tautenhahn
APPLICATION NOTE 73325
Demonstrate the LC-MS data dependent MSn workflow for the confident untargeted annotation of unknown metabolites and inclusion list-based identification of metabolite standards using a Thermo Scientific™ Orbitrap™ ID-X™ Tribrid™ mass spectrometer and Thermo Scientific™ Compound Discoverer™ software. Compare the results from standard mixtures, NIST SRM 1950 plasma and spiked plasma extracts to provide unequivocal evidence of highly confident identifications.


Thermo Fisher Scientific, San Jose, California, USA