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Metabolite analysis in Curcuma domestica using various GC-MS and LC-MS separation and detection techniques

Reputable Mentor II
Reputable Mentor II
Herebian D, Choi JH, Abd El-Aty AM, Shim JH, Spiteller M.
Biomed Chromatogr. 2009 Sep;23(9):951-65.
The metabolic profile of polar (methanol) and non-polar (hexane) extracts of Curcuma domestica, a widely used medicinal plant, was established using various different analytical techniques, including GC-FID, GC-MS, HR-GC-MS and analytical HPLC-ESI-MS/MS by means of LTQ-Orbitrap technology. The major non-volatile curcuminoids curcumin, demethoxycurcumin and bisdemethoxycurcumin were identified when their chromatographic and precursor ion masses were compared with those of authentic standard compounds. In this paper we describe for the first time a GC/MS-based method for metabolic profiling of the hydrophilic extract. We also identified 61 polar metabolites as TMS derivatives.
Institute of Environmental Research, University of Dortmund, Dortmund, Germany.
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