Stratton T, Ding C, Huang Y, Schaeffer D.
Application Note 564
Early screening of drug candidates for favorable in vitro metabolic stability can provide a window on potential pharmacokinetic performance and interspecies scaling and offer feedback to medicinal chemistry to improve upon compound series. Historically these screening assays used triple-stage quadrupole mass spectrometers, which provide good quantitative data but require compoundspecific tuning. Qualitative data was limited without significant additional method development. We present here the use of a benchtop high-resolution, accurate-mass mass spectrometer coupled with acquisition and processing software for simultaneous quantitative and qualitative (quan/qual) analysis of in vitro samples.
Thermo Fisher Scientific
United States
