on 08-09-201210:44 AM - edited on 10-15-202111:17 AM by Closed Account
Stratton T, Ding C, Huang Y, Schaeffer D. Application Note 564 Early screening of drug candidates for favorable in vitro metabolic stability can provide a window on potential pharmacokinetic performance and interspecies scaling and offer feedback to medicinal chemistry to improve upon compound series. Historically these screening assays used triple-stage quadrupole mass spectrometers, which provide good quantitative data but require compoundspecific tuning. Qualitative data was limited without significant additional method development. We present here the use of a benchtop high-resolution, accurate-mass mass spectrometer coupled with acquisition and processing software for simultaneous quantitative and qualitative (quan/qual) analysis of in vitro samples.