on 06-22-202004:36 AM - edited on 10-15-202111:20 AM by AnalyteGuru
Ioanna Ntai, Amanda Souza, Ralf Tautenhahn, and Andreas Hühmer,
Purpose: Monitor known metabolic differentiators and discover novel biomarkers of diet-induced obesity.
Methods: Plasma samples from 20 mice fed normal diet and 20 mice fed high-fat diet for 4 weeks were analyzed with a Thermo Scientific™ Vanquish™ UHPLC system and a Thermo Scientific™ Orbitrap Exploris™ 240 mass spectrometer and data were processed using Thermo Scientific™ Compound Discoverer™ software.
Results: A semi-targeted workflow was developed for the detection of known obesity markers, such as branched-chain amino acids, while at the same time, enabling comprehensive metabolic phenotyping of mouse plasma samples. Over 4,000 metabolites were detected. Both diet and sex contributed to modified metabolite levels as detected by differential analysis.