cancel
Showing results for 
Search instead for 
Did you mean: 
Orbitrap_SciLib
Reputable Mentor II
Reputable Mentor II
Sampaio JL, Gerl MJ, Klose C, Ejsing CS, Beug H, Simons K, Shevchenko A.
Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1903-7.
Tissue differentiation is an important process that involves major cellular membrane remodeling. We used Madin–Darby canine kidney cells as a model for epithelium formation and investigated the remodeling of the total cell membrane lipidome during the transition from a nonpolarized morphology to an epithelial morphology and vice versa. To achieve this, we developed a shotgun-based lipidomics workflow that enabled the absolute quantification of mammalian membrane lipidomes with minimal sample processing from low sample amounts. Epithelial morphogenesis was accompanied by a major shift from sphingomyelin to glycosphingolipid, together with an increase in plasmalogen, phosphatidylethanolamine, and cholesterol content, whereas the opposite changes took place during an epithelial-to-mesenchymal transition. Moreover, during polarization, the sphingolipids became longer, more saturated, and more hydroxylated as required to generate an apical membrane domain that serves as a protective barrier for the epithelial sheet.

http://www.pnas.org/content/108/5/1903.full.pdf
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Version history
Last update:
‎10-15-2021 12:03 PM
Updated by:
Closed Account
Contributors