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Reputable Mentor II
Reputable Mentor II
Graeme C. McAlister1, Christian Thoeing2, Helene L. Cardasis1, Alex S. Hebert3, Romain Huguet1, Philip Remeš1, Andreas Kuehn2, Mike Senko1, Shannon Eliuk1, Joshua J. Coon3, Vlad Zabrouskov1
ASMS 2017 Poster
Thermo Scientific™ Tribrid™ mass spectrometers are incredibly versatile instruments that combine multiple empowering MS technologies into a single platform. Working together, these technologies can sequence tens of thousands of peptides during a data-dependent LC-MS/MS analysis of a complex peptidic sample. To push sampling depths even further, we have deployed a new peak determination algorithm that identifies hundreds of thousands of additional precursors. To better sample all these additional precursors, we configured the ion trap to collect MS2 spectra at up to 40 Hz. Together these changes allow us to collect hundreds of thousands of MS2 spectra, which translates into >35% more unique peptide identifications.

1. Thermo Fisher Scientific, San Jose, CA 2. Thermo Fisher Scientific, Bremen, Germany 3. University of Wisconsin, Madison, WI
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‎10-15-2021 11:32 AM
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