Showing results for 
Search instead for 
Did you mean: 

Mass spectrometry for proteomics

Reputable Mentor II
Reputable Mentor II
Han X, Aslanian A, Yates JR 3rd.
Curr Opin Chem Biol. 2008 Oct;12(5):483-90.
Mass spectrometry has been widely used to analyze biological samples and has evolved into an indispensable tool for proteomics research. Our desire to understand the proteome has led to new technologies that push the boundary of mass spectrometry capabilities, which in return has allowed mass spectrometry to address an ever-increasing array of biological questions. The recent development of a novel mass spectrometer (Orbitrap) and new dissociation methods such as electron-transfer dissociation has made possible the exciting new areas of proteomic application. Although bottom-up proteomics (analysis of proteolytic peptide mixtures) remains the workhorse for proteomic analysis, middle-down and top-down strategies (analysis of longer peptides and intact proteins, respectively) should allow more complete characterization of protein isoforms and post-translational modifications. Finally, stable isotope labeling strategies have transformed mass spectrometry from merely descriptive to a tool for measuring dynamic changes in protein expression, interaction, and modification.
Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrev Pines Road, La Jolla, CA 92037, USA.
Version history
Last update:
‎10-15-2021 12:08 PM
Updated by: