on 05-01-201210:46 AM - edited on 10-15-202111:40 AM by AnalyteGuru
Ting L, Rad R, Gygi SP, Haas W. Nat Methods. 2011 Oct 2;8(11):937-40. Quantitative mass spectrometry-based proteomics is highly versatile but not easily multiplexed. Isobaric labeling strategies allow mass spectrometry-based multiplexed proteome quantification; however, ratio distortion owing to protein quantification interference is a common effect. We present a two-proteome model (mixture of human and yeast proteins) in a sixplex isobaric labeling system to fully document the interference effect, and we report that applying triple-stage mass spectrometry (MS3) almost completely eliminates interference.