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MS3 eliminates ratio distortion in isobaric multiplexed quantitative proteomics

Reputable Mentor II
Reputable Mentor II
Ting L, Rad R, Gygi SP, Haas W.
Nat Methods. 2011 Oct 2;8(11):937-40.
Quantitative mass spectrometry-based proteomics is highly versatile but not easily multiplexed. Isobaric labeling strategies allow mass spectrometry-based multiplexed proteome quantification; however, ratio distortion owing to protein quantification interference is a common effect. We present a two-proteome model (mixture of human and yeast proteins) in a sixplex isobaric labeling system to fully document the interference effect, and we report that applying triple-stage mass spectrometry (MS3) almost completely eliminates interference.
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
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