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Low pg/mL Detection of rHuEPOs in Horse Plasma Employing High-Resolution MS

Reputable Mentor II
Reputable Mentor II

Application Note 585
Recombinant human erythropoietin (rHuEPO) is a 30–34 kDa glycoprotein and a common doping agent banned by equestrian and racing authorities. To differentiate between equine endogenous EPO and rHuEPOs, methods employing selected-reaction monitoring (SRM) or isoelectric focusing (IEF) with double-blotting have been developed. However, the short confirmation-time window of the compound in plasma continues to present a challenge for doping control laboratories in horse racing. In this study, a new sample preparation technique using small anti-EPO monolith membranes contained in disposable columns (anti-EPO monolith columns) was combined with nanoLC-MS/MS analysis on a benchtop high-resolution Thermo Scientific™ Orbitrap™-based mass spectrometer. The approach enabled the confirmation and quantitation of the rHuEPO target peptides in a single analytical run, with high specificity, sensitivity, and resolution.

Thermo Fisher Scientific
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Last update:
‎10-15-2021 09:03 AM
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