Huguet R, Blank M, Soltero N, Sharma S, Zabrouskov.
ASMS 2015 Poster Note 64492
Purpose: Demonstrate improved limit of quantification (LOQ) possible for the new Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer utilizing several targeted and data independent (DIA) methods for peptide quantitation.
Methods: A set of stable isotope-labeled synthetic peptides were spiked in various concentrations into high complexity matrices. They were analyzed using high resolution accurate mass (HRAM) MS1/MS2 DIA methods and HRAM MS2 targeted methods.
Results: Limit of quantitation (LOQ) down to 1 attomole (CV<15%), while still maintaining linearity, was observed when using the targeted strategy which proved to be the most sensitive of all the tested techniques. Several orders of dynamic range were observed from the quantitation results of the DIA methods which exhibited limits of quantification down to 100 attomoles.
Thermo Fisher Scientific
United States
