on 05-01-201210:41 AM - edited on 10-15-202111:46 AM by AnalyteGuru
Nygren H, Seppänen-Laakso T, Castillo S, Hyötyläinen T, Orešič M. Methods Mol Biol. 2011;708:247-57. In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultra-high performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MSn mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses as is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.