on 08-24-201204:51 AM - edited on 10-15-202111:09 AM by AnalyteGuru
Qiao X, Tao D, Qu Y, Sun L, Gao L, Zhang X, Liang Z, Zhang L, Zhang Y. Proteomics. 2011 Nov;11(21):4274-8. The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF(4)) and those in soluble fraction, a total number of 462 non-redundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers.