Ryan D. Bomgarden, Amarjeet Flora, Sergei Snovida, Penny Jensen, John C. Rogers
ASMS 2019 Poster
Phosphorylation is a critical post translational modification that modulates the function of numerous proteins, and recent advances in mass spectrometry (MS) instrumentation have enabled studying phosphorylation at proteome- wide scale in complex biological samples. However, due to the low abundance of phosphorylated peptides in protein digests, affinity-based phosphopeptide enrichment from milligrams of protein digest is required for MS detection and quantification. Recently, we developed a new, simplified sample prep kit containing pre-formulated reagents and a standardized protocol for processing 10 to 100µg protein samples in less than 2 hours. In this study, we assessed the scalability of our chemistry on larger protein amounts (>1mg) for subsequent phosphopeptide enrichment using immobilized metal affinity chromatography (IMAC).
Thermo Fisher Scientific, Rockford, IL, USA, 61101
United States
