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Large Scale EasyPep MS Sample Preparation for Phosphopeptide Enrichment Workflows

Reputable Mentor II
Reputable Mentor II
Ryan D. Bomgarden, Amarjeet Flora, Sergei Snovida, Penny Jensen, John C. Rogers
ASMS 2019 Poster
Phosphorylation is a critical post translational modification that modulates the function of numerous proteins, and recent advances in mass spectrometry (MS) instrumentation have enabled studying phosphorylation at proteome- wide scale in complex biological samples. However, due to the low abundance of phosphorylated peptides in protein digests, affinity-based phosphopeptide enrichment from milligrams of protein digest is required for MS detection and quantification. Recently, we developed a new, simplified sample prep kit containing pre-formulated reagents and a standardized protocol for processing 10 to 100µg protein samples in less than 2 hours. In this study, we assessed the scalability of our chemistry on larger protein amounts (>1mg) for subsequent phosphopeptide enrichment using immobilized metal affinity chromatography (IMAC).

Thermo Fisher Scientific, Rockford, IL, USA, 61101
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Last update:
‎11-09-2021 01:15 AM
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