on 05-01-201210:54 AM - edited on 10-15-202111:25 AM by Closed Account
Dutta S, Delvolve A, Jackson S, Airavaara M, Harvey B, Wang Y, Woods A. Scientific Poster Apart from water, lipids are the most common biomolecules found in the brain (12 %) and make up 50% of its dry weight. Furthermore, lipids are the major building blocks of biomembranes; play a key role in signal transduction, and are an important reservoir of energy in biological systems. Additionally, changes in the levels of lipids, in particular ceramides and glycerophospholipids, have been observed in apoptosis or cell death.
Lipids represent a large and very diverse group of biomolecules that have one or both of the following properties: soluble in organic solvents and the presence of long hydrocarbon chains. Lipids can be organized into eight categories: fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterol lipids, prenol lipids, saccharolipids, and polyketides. Glycerophospholipids are widely abundant in nature, especially in biological membranes, and are divided into classes based upon their head group as follows: glycerophosphocholines (PCs), glycerophosphoethanolamines (PEs), glycerophosposerines (PSs), glycerophosphoglycerols (PGs), glycerolphosphoinositols (PIs), and glycerophosphates (PAs). Sphingolipids contain a sphingoid base backbone and include sphingomyelins (SMs), sulfatides (STs), ceramides, cerebrosides, and gangliosides.
In this study, LC ESI MS and MALDI-MS will be used to analysis the lipidome for an animal model of stroke with particular focus on glycerophospholipids and sphingolipids.