LC-MS quantitative screening method for 18 anabolic steroids in oral fluid using MS2 spectra data collected with Q Exactive Orbitrap mass spectrometer

[Orbitrap_SciLib]

Kozak M.
Scientific Poster
Purpose: To develop a sensitive method for quantitative screening of 18 anabolic steroids in oral fluid. Methods: Samples were processed with LLE, analyzed with a 15 min. LC gradient, and compounds were identified with ion ratio calculated for fragments in MS2 spectrum Results: The LLOQ was 1ng/mL for all analytes except for 6β-Hydroxyfluoxymesterone (6 ng/mL). The UPLQ was between 60-1500 ng/mL, and it was lower for compounds producing high signal in mass spectrometer detector. Matrix effects were not observed: percent recovery in spiked blank oral fluid and analyzed with calibration standards prepared in solvent was in range 78.5-118%.


Thermo Fisher Scientific