on 03-13-201307:47 AM - edited on 10-15-202108:03 AM by AnalyteGuru
Kozak M. Scientific Poster Purpose: To develop a sensitive method for quantitative screening of 18 anabolic steroids in oral fluid.
Methods: Samples were processed with LLE, analyzed with a 15 min. LC gradient, and compounds were identified with ion ratio calculated for fragments in MS2 spectrum
Results: The LLOQ was 1ng/mL for all analytes except for 6β-Hydroxyfluoxymesterone (6 ng/mL). The UPLQ was between 60-1500 ng/mL, and it was lower for compounds producing high signal in mass spectrometer detector. Matrix effects were not observed: percent recovery in spiked blank oral fluid and analyzed with calibration standards prepared in solvent was in range 78.5-118%.