Intelligent Acquisition for Comprehensive Metabolome Coverage in Plants, Mammals, and Bacteria

[Orbitrap_SciLib]

Tatjana D. Talamantes, Sven Hackbusch, Ioanna Ntai, Amanda L. Souza

Purpose: Demonstration of an intelligent data acquisition workflow used for comprehensive coverage regardless of sample type and complexity using real-time data reduction. Methods: Multiple types of samples with varying complexity (human plasma, urine, E. coli, yeast and green tea extract) were analyzed with a Thermo Scientific™ Orbitrap ID-X™ Tribrid™ mass spectrometer using Thermo Scientific™ AcquireX intelligent data acquisition software. Data were evaluated using Thermo Scientific™ Compound Discoverer™ software with the mzCloud™ spectral library. Results: By excluding background and degenerate signals, the total number of fragmentation targets was reduced without compromising metabolite coverage thus allowing for acquisition to focus on biologically relevant compounds and demonstrably increase the depth of MS/MS coverage.


Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, CA, USA, 95134