1) Institute of Nutritional Physiology ("Oskar Kellner"), Leibniz Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany, 2) Institute of Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany, 3) Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, 53113 Bonn, Germany, 4) Veterinary Physiology, Vetsuisse Faculty, University of Bern, 3001 Bern, Switzerland Journal of Dairy Science (2016), In press, doi: 10.3168/jds.2016-11022 Insulin-dependent glucose metabolism in dairy cows with variable fat mobilization around calving Weber C. (1), Schäff C.T. (1), Kautzsch U. (1), Börner S. (1), Erdmann S. (1), Görs S. (1), Röntgen M. (2), Sauerwein H. (3), Bruckmaier R.M. (4), Metges C.C. (1), Kuhla B. (1) and Hammon H.M. (1) Dairy cows undergo significant metabolic and endocrine changes during the transition from pregnancy to lactation, and impaired insulin action influences nutrient partitioning toward the fetus and the mammary gland. Because impaired insulin action during transition is thought to be related to elevated body condition and body fat mobilization, we hypothesized that over-conditioned cows with excessive body fat mobilization around calving may have impaired insulin metabolism compared with cows with low fat mobilization.