Increased confidence of insect lipidome annotation from high-resolution Orbitrap LC/MSn analysis and LipidSearch software

[Orbitrap_SciLib]

David Peake1, Reiko Kiyonami1, Yasuto Yokoi2, Yelena Aldefinskaya3, Jeff Gilbert3, and Daniel Gachotte3
APPLICATION NOTE 72942
• Demonstrate the utility of LC/MS and data-dependent MSn acquisition for simultaneous lipid profiling and more complete lipid annotation using the Thermo Scientific™ Orbitrap™ ID-X™ Tribrid™ mass spectrometer and Thermo Scientific™ LipidSearch™ software. • Show that the use of the Thermo Scientific™ AcquireX intelligent acquisition method combined with neutral loss and product ion scan filters increases the overall quality and number of lipid annotations. • Investigate the utility of combining HCD MS2 and CID MSn for structural determination of phosphatidylcholine and triglyceride species in lipid extracts from insect larvae.


1Thermo Fisher Scientific, San Jose, California, USA 2Mitsui Knowledge Industry Co., LTD, Tokyo, Japan 3CORTEVA agriscience, Indianapolis, Indiana, USA