on 08-08-201808:06 AM - edited on 10-15-202108:20 AM by AnalyteGuru
Daniel Gachotte1, Yelena Adelfinskaya1, Jeffrey Gilbert1, Vimbai Chikwana1, Sek Yee Tan1, Jonathan Robeson2, Adane Kassa2, Ken Narva1, Reiko Kiyonami3, David Peake3 and Yasuto Yokoi4
ASMS 2018 Insect lipids are highly structurally diverse species that perform many important functions including storage of metabolic energy, contributing to the structure of membranes, protection against dehydration and pathogens, and circulating energy molecules. In addition, essential lipids such as sterols (precursor of hormone) and polyunsaturated fatty acids are only available from the diet. Understanding how lipid molecular species change in response to both diet and age is critical to define nutrient requirement and fitness relationship. Detailed studies of insect lipid composition however, have been technically challenging because of the complexity of lipid profiles. We evaluated the robustness of a new high resolution LC-MSn approach to perform non-targeted lipid profiling experiments. Western Corn Rootworm larvae (Diabrotica virgifera virgifera) was chosen due to it’s economical impact estimated at 2 Billions US$ cost associated with its control and corn production loss in the Americas. Three populations of larvae per condition were extracted and the lipids were mixed with commercially available standards. Several data acquisition modes were tested but only the AcquireX™ is presented in this work.
1. Dow AgroSciences, Indianapolis, IN
2. Pioneer, Johnston,IA
3. Thermo Fisher Scientific, San Jose, CA
4. Mitsui Knowledge Industry, Tokyo, Japan