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In-Depth Proteome Coverage by Iterative Data Dependent Acquisition on a Benchtop Orbitrap Mass Spectrometer

Reputable Mentor II
Reputable Mentor II
Mueller M, Kuehn A, Xuan Y, Arrey T, Rietpietsch T, Grosse-Coosmann F, Crone C, Ueckert T, Kellmann M.
ASMS 2015 Poster Note 64431
Purpose: Increase the dynamic range for protein identification in complex proteome samples, by applying an iterative acquisition strategy. Methods: After each LC-MS/MS run the data scenario is analyzed and a strategy regarding LC and MS parameters for a next iteration is determined, based on potential precursor candidates, their timely distribution and ion flux trends. Hereby, already triggered precursors are excluded, while missed candidates are included as targets of interest. Results: Monitoring the time and flux distribution of available precursors over LC-MS/MS runs can be used to adapt sample loads for iterative LC-MS/MS runs to stay at a high sequencing speed with a maximized number of selectable precursors.

Thermo Fisher Scientific
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Last update:
‎10-15-2021 11:34 AM
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