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In-Depth Characterization of Intact Protein Standards Using HRAM Top Down Mass Spectrometry with Multiple MSMS Strategies

Reputable Mentor II
Reputable Mentor II

Helene Cardasis1, Romain Huguet1, Chris Mullen1, Stephane Houel1, Luca Forneli2, Rosa Viner1, Viktorija Vitkovske1, Shanhua Lin1, Seema Sharma1, Vlad Zabrouskov1, Neil Kelleher2
ASMS 2017 Poster
Purpose: Demonstrate unique characteristics and effectiveness of various dissociation mechanisms for intact protein identification and characterization. Methods: Collection and analysis of high resolution CID, HCD, ETD, and UVPD data on various proteins at various energies or reaction times. Results: Each fragmentation mechanism generates unique data that, together, maximizes sequence coverage for improved protein identification and proteoform characterization. Considerations for optimizing each dissociation mechanism with respect to proteins representing a MW range from 9kDa to 50kDa are presented.

1. Thermo Fisher Scientific, San Jose, CA USA 2. Northwestern University, Chicago, IL USA

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‎03-23-2022 06:58 AM
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