In-Depth Characterization of Intact Protein Standards Using HRAM Top Down Mass Spectrometry with Multiple MSMS Strategies

[Orbitrap_SciLib]

Helene Cardasis1, Romain Huguet1, Chris Mullen1, Stephane Houel1, Luca Forneli2, Rosa Viner1, Viktorija Vitkovske1, Shanhua Lin1, Seema Sharma1, Vlad Zabrouskov1, Neil Kelleher2
ASMS 2017 Poster
Purpose: Demonstrate unique characteristics and effectiveness of various dissociation mechanisms for intact protein identification and characterization. Methods: Collection and analysis of high resolution CID, HCD, ETD, and UVPD data on various proteins at various energies or reaction times. Results: Each fragmentation mechanism generates unique data that, together, maximizes sequence coverage for improved protein identification and proteoform characterization. Considerations for optimizing each dissociation mechanism with respect to proteins representing a MW range from 9kDa to 50kDa are presented.


1. Thermo Fisher Scientific, San Jose, CA USA 2. Northwestern University, Chicago, IL USA