on 05-15-201208:47 AM - edited on 11-09-202101:47 AM by usermigration2
Zhang T, Herbold R, Comstock K, Huang Y, Jiang G. ASMS 2012 Poster Purpose: Develop a simple assay for accurate and robust impurity analysis of carbamazepine using the Accela UHPLC system and a high performance Hypersil GOLD CN (cyano) column. And combining the high resolution, high accurate mass spectrometry and Mass Frontier software is a powerful solution for definitive identification and structural confirmation of drug impurities.
Methods: HPLC separations were performed on an Accela 1250 LC system and an Accela autosampler (Thermo Fisher Scientific, San Jose, CA, USA). UV absorbance was monitored at 211 nm at 20 Hz using an Accela PDA detector (Thermo Fisher Scientific, San Jose, CA, USA). For LC-MS analysis the LC set up was coupled to an Orbitrap Velos Pro.
Results: A simple, accurate and robust quantitative HPLC/UV assay for carbamazepine purity assessment was developed. A high-resolution, accurate mass LC-MS method was used for identification and confirmation of impurities in carbamazepine drug substance.