on 05-15-201211:14 PM - edited on 11-09-202101:47 AM by usermigration2
Prieto Conaway MC, Sutton J, Stratton T, Wang L, Comstock K, Hok S, Balhorn R. ASMS 2012 Poster Purpose: Impurity analysis using LC-MS and differential analysis software to determine impurities between two synthetic pathways for a drug with potential in treating Non- Hodgkin’s Lymphoma.
Methods: A bench-scale synthesis of the drug is compared to the first batch of a pilot production synthesis. LC-MS (positive mode) using an Open Accela Autosampler coupled to a Thermo Scientific LTQ Orbitrap XL mass spectrometer followed by SIEVE™ 2.0 software analysis. Thermo Scientific Mass Frontier 7.0 software used for determination of fragments and fragmentation pathways.
Results: High resolution spectra and ion trap MSn data was used to elucidate an abundant impurity present in both products as a chromium chelate to the DOTA structure. Differential analysis software identified a number of differences between the two samples. The extracted ion chromatograms were reconstructed from the HRAM data and elucidation of impurities was performed by ion trap MSn and Mass Frontier™ software.