on 05-01-201210:54 AM - edited on 10-15-202112:07 PM by Closed Account
Damoc E, Denisov E, Lange O, Moehring T, Makarov A. ASMS 2011 Poster Major goals in every top-down proteomics experiment are protein identification and characterization. The strategy used to achieve these goals involves high-resolution mass measurement of the molecular weight of the intact protein and fragment ions. In spite of enormous improvements in terms of speed and sensitivity in the FTMS instrumentation over the last few years, top-down LC-MS/MS in current large scale proteome analyses remains limited. Further improvement of higher-resolution analysis at faster detection speed is required to advance the development of proteome analyses. Recent innovations in the Orbitrap technology, such as high-field Orbitrap mass analyzer and advanced signal processing1, implemented in the new Thermo Scientific Orbitrap Elite mass spectrometer, accelerated detection by around 3.6-fold.