Improving high-throughput Top-Down proteomics using a modified hybrid quadrupole-ultra-high-field-Orbitrap mass spectrometer

[Orbitrap_SciLib]

Eugen Damoc1, Kyle L. Fort2, Michiel van de Waterbeemd2, Sem Tamara2, Christian Thoeing1, Erik Couzijn1, Kai Scheffler3, Tabiwang Arrey1, Alexander Harder1, Albert J. R. Heck2 and Alexander Makarov1
ASMS 2017 Poster
Purpose: Demonstrate improved Top-Down performance using a Thermo Scientific™ Q Exactive™ HF-X mass spectrometer and advanced Data-Dependent Acquisition (DDA) algorithms Methods: Improved ‘Medium-High’ and ‘High-High’ Top-Down DDA methods Results: Redundant fragmentation is significantly reduced and more proteoforms are identified using the Q Exactive HF-X mass spectrometer and improved Top-Down DDA methods


1 Thermo Fisher Scientific (Bremen) GmbH, Hanna-Kunath-Str. 11, 28199 Bremen, Germany 2 Utrecht University, Padualaan 8, The Netherlands 3 Thermo Fisher Scientific GmbH, Im Steingrund 4-6, 63303 Dreieich, Germany