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Improving Intact Antibody Characterization by Orbitrap Mass Spectrometry

Reputable Mentor II
Reputable Mentor II
Scheffler K, Damoc E, Muller M, Zeller M, Moehring T
BioPharma_PN63712_E 11/13S
Here we present an approach to analyze an intact monoclonal antibody in non-reduced and reduced condition by LC-MS using the Thermo Scientific™ Orbitrap Elite™ mass spectrometer. The intact antibody and the separated light and heavy chains were analyzed in Full MS experiments as well as with top-down experiments using in-source CID (SID), CID, HCD and ETD fragmentation techniques making use of the ultrahigh resolution of the mass spectrometer. For data evaluation ProSight software and Thermo Scientific™ Protein Deconvolution™ software version 1.0 packages were used.

ThermoFisher Scientific
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Last update:
‎10-15-2021 12:05 PM
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