Scheffler K, Damoc E, Muller M, Zeller M, Moehring T
BioPharma_PN63712_E 11/13S
Here we present an approach to analyze an intact monoclonal antibody in non-reduced and reduced condition by LC-MS using the Thermo Scientific™ Orbitrap Elite™ mass spectrometer. The intact antibody and the separated light and heavy chains were analyzed in Full MS experiments as well as with top-down experiments using in-source CID (SID), CID, HCD and ETD fragmentation techniques making use of the ultrahigh resolution of the mass spectrometer. For data evaluation ProSight software and Thermo Scientific™ Protein Deconvolution™ software version 1.0 packages were used.
ThermoFisher Scientific
United States
