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Improving ETD Analysis of N, O-glycopeptides by Using the Isobaric Labeling Approach with Tandem Mass Tags (TMT)

Reputable Mentor II
Reputable Mentor II
Zhang T, Viner R, Zabrouskov V
Scientific Poster
Electron transfer dissociation (ETD) preserves labile post-translational modifications (PTMs) and allows direct mapping of peptide/protein modifications which made this type of mass spectrometry fragmentation the method of choice for sequencing of N-, O-glycopeptides. However to make ETD successful high charge state precursor ions (3 and above) are preferred. In the case of N-glycopeptides, the presence of multiple negatively charged sialic acid residues presents additional challenge as it significantly reduces the average precursor charge state. In this study we 1) investigated the effect of the addition of the basic Tandem Mass Tag (TMTTM) groups to increase average precursor charge state and as a result to improve ETD fragmentation and 2) attempted relative quantification of TMT labeled glycopeptides by ETD.

Thermo Fisher Scientific
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Last update:
‎10-15-2021 11:36 AM
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