on 06-12-201803:22 PM - edited on 10-15-202111:30 AM by AnalyteGuru
Ioanna Ntai, Iman Mohtashemi, Jenny Berryhill, Ralf Tautenhahn, Graeme McAlister, Derek Bailey, Linda Lin, Ryo Komatsuzaki, Caroline Ding, Seema Sharma, Tim Stratton, Vlad Zabrouskov, Amanda Souza, Andreas Huhmer ASMS 2018 Purpose: Develop a workflow that maximizes the number of unique metabolites interrogated by MS/MS and MSn, while minimizing the acquisition of uninformative data, resulting in comprehensive metabolome coverage.
Methods: Human plasma (NIST SRM1950) was analyzed with a Thermo Scientific™ Orbitrap ID-X™ Tribrid™ mass spectrometer. The data were analyzed using Thermo Scientific™ Compound Discoverer™ 3.0 software for metabolite identification.
Results: The Orbitrap ID-X Tribrid MS with AcquireX resulted in more unique precursor ions
fragmented and consequently more metabolites identified in human plasma.