Improved Identification, Quantification Accuracy, and Workflow Efficiency using a Modified Quadrupole Orbitrap Mass Spectrometer and Tandem Mass Tags (TMT) Approach

[Orbitrap_SciLib]

Aaron M. Robitaille 1, Tabiwang N. Arrey 2, Markus Kellmann 2, Arne Kreutzmann 2, Daniel Mourad 2, Daniel Lopez Ferrer 1, Andreas Huhmer 1, Alexander Harder 2

Multiplexed quantitation strategies using Thermo Scientific™ Tandem Mass Tags™ (TMT™) enable precise measurement of peptide or protein abundance from multiple samples into a single high-resolution LC-MS analysis. Increasingly, various biological experiments demand higher quantitation accuracy and proteome coverage. Here, we demonstrate increased quantitative performance and throughput efficiency on a Thermo Scientific™ Orbitrap Exploris™ 480 mass spectrometer combined with a Thermo Scientific™ FAIMS Pro™ interface.


1 Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, CA 95134. 2 Thermo Fisher Scientific Hanna-Kunath-Straße 11, 28199 Bremen, Germany