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Improved Detection of Proteins in Complex Sample Matrices by Infusion Using FAIMS

Reputable Mentor II
Reputable Mentor II
Abbatiello S, Cardasis H, Belford M, Sarracino D, Neil J, Stephenson J, Dunyach J, Blackburn M.
ASMS 2015 Poster Note 64473
Purpose: Evaluate FAIMS for the improved detection of proteins by infusion. Methods: Protein standards and samples (from cell lysates or digested plasma) were infused at 3 uL/min using FAIMS on a Thermo ScientificTM Orbitrap Velos ProTM mass spectrometer. Compensation voltages were stepped to promote separation of analytes from high-intensity singly charged contaminant and other sample-related background ions. Results: Detection of protein ions by infusion was drastically improved using FAIMS. Singly charged contaminant ions were preferentially excluded during mass detection, resulting in significantly improved signal-to-noise of over 50 proteins in a cell lysate. Detection of insulin was also improved versus no FAIMS, resulting in simplified, chromatography-free sample introduction.

Thermo Fisher Scientific
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Last update:
‎10-15-2021 12:04 PM
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