Abbatiello S, Cardasis H, Belford M, Sarracino D, Neil J, Stephenson J, Dunyach J, Blackburn M.
ASMS 2015 Poster Note 64473
Purpose: Evaluate FAIMS for the improved detection of proteins by infusion.
Methods: Protein standards and samples (from cell lysates or digested plasma) were
infused at 3 uL/min using FAIMS on a Thermo ScientificTM Orbitrap Velos ProTM mass spectrometer. Compensation voltages were stepped to promote separation of analytes from high-intensity singly charged contaminant and other sample-related background ions.
Results: Detection of protein ions by infusion was drastically improved using FAIMS.
Singly charged contaminant ions were preferentially excluded during mass detection,
resulting in significantly improved signal-to-noise of over 50 proteins in a cell lysate.
Detection of insulin was also improved versus no FAIMS, resulting in simplified,
chromatography-free sample introduction.
Thermo Fisher Scientific
United States
