on 06-20-201712:14 AM - edited on 10-15-202108:42 AM by AnalyteGuru
Christian Thoeing, Sebastien Gallien, Tabiwang Arrey, Yue Xuan, Oliver Lange, Kerstin Strupat , Markus Kellmann
Purpose: On-the-fly correction of scheduled retention time windows in order to allow more reliable profiling of more peptide targets
Methods: Dynamic retention time window correction for scheduled PRM was applied fully unattended and automated on a Thermo Scientific™ Q Exactive™ HF-X hybrid quadrupole-Orbitrap™ mass spectrometer.
Results: The dynamic correction of scheduled retention time windows in nano-LC setups allows for decreasing retention time windows to 60 seconds with increased reliability compared to static retention time window scheduling. This reduces the degree of overlapping of retention time windows, which in turn increases the amount of peptide targets that can be scheduled in an experiment.