Identifying Quantitative Protein Changes in Iris Biopsies of Glaucoma Patients Using Label Free Proteomics

[Orbitrap_SciLib]

Craig Dufresne1, Richard Semba2, Pingbo Zhang2, Min Zhu2, Jiang Qian3, Tianshun Gao3, Ibrahim AlJadaan4, Sami AlShahwan4, Ohood Owaidha4, Randy Craven2, Deepak Edward2,4, Alka Mahale4;  

Purpose: Label free quantification (LFQ) of iris biopsies from patients with primary angle closure glaucoma (PACG) compared to healthy eyes. Methods: Quadrupole-Orbitrap mass spectrometry quantitative proteomics. Results: LFQ identified differences in protein concentrations between PACG and control iris samples, providing new insights into pathogenesis of glaucoma.


1Thermo Fisher Scientific, West Palm Beach, FL, United States; 2Johns Hopkins University, Baltimore, MD, United States; 3National Institute on Aging, National Institutes of Health, Baltimore, MD, United States; 4King Khaled Eye Specialist Hospital, Riyadh, Central, Saudi Arabia