on 11-10-201503:28 PM - edited on 10-15-202110:57 AM by Closed Account
Xiaoyue Jiang and Rosa Viner
A presentation providing an overview of multiplexed isobaric labeling, the basics of Tandem Mass Tags and the SPS MS3 technique. The presentation also covers sample prep. Specifically summary of the steps involved for a complete TMT workflow including labeling and fractionation. The third part of the presentation will show instruments that are used in this workflow. How to get started with building an instrument method on the Orbitrap Tribrids and Benchtops mass pectrometers. Final part will cover data Analysis. Data analysis of SPS MS3 data using Proteome Discoverer 2.1.