on 03-09-201702:43 AM - edited on 11-09-202101:27 AM by usermigration2
Wei Jia a, b *, Xiaogang Chu a, b, James Chang c, Perry G. Wang d, Ying Chen b, Feng
Zhang b W. Jia, X. Chu, J. Chang, P.G. Wang, Y. Chen, F. Zhang, High-throughput untargeted screening of veterinary drug residues and metabolites in tilapia using high resolution orbitrap mass spectrometry, Analytica Chimica Acta (2017), doi: 10.1016/j.aca.2016.12 A analytical method was developed and validated for simultaneous analysis of one hundred and thirty-seven veterinary drug residues and metabolites from sixteen different classes in tilapia utilizing an improved fully non-targeted way of data acquisition with fragmentation. The
automated on-line extraction procedure was achieved in a simple disposable pipet extraction.
Ultrahigh-performance liquid chromatography and electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC Q-Orbitrap) was used for the separation and detection of all the analytes. The methodology was validated by taking into consideration the
guidelines specified in European SANCO/12571/2013 Guideline 2013 and Commission Decision 2002/657/EC. The extraction recoveries ranged from 81% to 111%. The limits of decision ranged from 0.01 to 2.73 μg kg-1 and the detection capabilities ranged from 0.01 to 4.73 μg kg-1. The one hundred and thirty-seven compounds behave dynamic 0.1-500 μg kg-1, with correlation coefficient > 0.99. The fully non-targeted data acquisition way improves both sensitivity and selectivity for the fragments, which is beneficial for screening performance and identification
capability. This validated method has been successfully applied on screening of veterinary drug residues and metabolites in muscle of tilapia, an important and intensively produced fish in aquaculture.
http://pubs.acs.org/doi/pdf/10.1021/acs.jafc.6b05034 a School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China
b Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China
c Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, California 95134, United States
d U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5100 Paint
Branch Parkway, HFS-717, College Park, MD 20740-3835, United States