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Orbitrap_SciLib
Reputable Mentor II
Reputable Mentor II
Khatereh Motamedchaboki1 , Maowei Dou1 , Yongzheng Cong2 , Romain Huguet1 , Aaron M Robitaille1 , Yufeng Shen3 , Geremy Clair4 ,Charles Ansong4 , Daniel Lopez-Ferrer1 , Ryan T. Kelly2,4, and Ying Zhu4
APPLICATION NOTE 65714
Understanding heterogeneity at the single cell level is of great interest for biomedical research. Mass spectrometry (MS) based proteomics is a promising technique for single cell analysis by enabling the identification and quantification of thousands of proteins in an unbiased manner. However, due to inefficiencies in single cell isolation, large sample losses during sample preparation and low throughput, the extension of proteomics to single cell studies has been largely ineffective. To address these challenges, we combined nanoPOTS (Nanodroplet Processing in One-pot for Trace Samples) technology with Thermo Scientific™ Tandem Mass Tag™ (TMT™) isobaric labeling to efficiently process and analyze single mammalian cells containing <0.2 ng total proteins using the Thermo Scientific™ Orbitrap Eclipse™ Tribrid™ mass spectrometer with Real-Time Search to improve single cell proteome coverage and enhance quantification accuracy


1 Thermo Fisher Scientific, San Jose, CA, 2 Brigham Young University, Provo, UT, 3 CoAnn Technologies LLC, Richland, WA, 4 Pacific Northwest National Laboratory, Richland, WA, USA
Version history
Last update:
‎10-15-2021 10:53 AM
Updated by:
AnalyteGuru
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