on 10-22-201204:06 AM - edited on 10-15-202105:24 AM by Closed Account
Khandurina J, Tremblay P, Duczak N. ASMS 2011 Poster 1,4-Butanediol (BDO) is an important commodity chemical used to manufacture over 2.5 million tons of valuable polymers annually. It is currently produced exclusively through petrochemical routes from oiland natural gas. A systems-based metabolic engineering strategy was implemented to construct a strain of E. coli capable of producing high levels of bio-BDO from renewable carbohydrate feedstocks. To ensure efficient optimization of BDO production, high throughput screening methods to monitor the formation of BDO, key metabolic intermediates and by-products are required. Traditional LCMS approaches cannot sufficiently accommodate throughput demands. In this research, a LDTD APCI source directly coupled to a mass spectrometer (both triple quadrupole and Orbitrap based) was evaluated to fulfill speed and versatility requirements for this application.