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High throughput screening of 1,4-butanediol production in fermentation samples using a LDTD APCI ionization source coupled to a benchtop Orbitrap MS

Reputable Mentor II
Reputable Mentor II
Khandurina J, Tremblay P, Duczak N.
ASMS 2011 Poster
1,4-Butanediol (BDO) is an important commodity chemical used to manufacture over 2.5 million tons of valuable polymers annually. It is currently produced exclusively through petrochemical routes from oiland natural gas. A systems-based metabolic engineering strategy was implemented to construct a strain of E. coli capable of producing high levels of bio-BDO from renewable carbohydrate feedstocks. To ensure efficient optimization of BDO production, high throughput screening methods to monitor the formation of BDO, key metabolic intermediates and by-products are required. Traditional LCMS approaches cannot sufficiently accommodate throughput demands. In this research, a LDTD APCI source directly coupled to a mass spectrometer (both triple quadrupole and Orbitrap based) was evaluated to fulfill speed and versatility requirements for this application.
Genomatica Inc., San Diego, CA
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‎10-15-2021 05:24 AM
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