Mick Greer1, Vincent Gerbasi2, Joseph Greer3, Ryan Fellers3, David Horn4, Romain Huguet4, Susan Abbatiello5, Michael Belford4, Philip Compton2, Kenneth Durbin3, Neil Kelleher2, Richard LeDuc3, Scott Peterman4, Paul Thomas2
Purpose: Evaluate Thermo Scientific™ ProSightPD™ nodes for analysis of top-down FAIMS data.
Methods: Samples were fractionated offline prior to LC separation coupled to a FAIMS enabled Orbitrap mass spectrometer. RAW files were imported directly into Thermo Scientific™ Proteome Discoverer™ 2.3 for analysis using ProSightPD™ 2.0 nodes.
Results: ProSightPD software supports analysis of FAIMS separated top-down data collected at multiple CVs. Inclusion of FAIMS separation improved several top-down experimental metrics.
1 Thermo Fisher Scientific, Austin, TX,
2 Northwestern University, Evanston, IL
3 Proteinaceous Inc., Evanston, IL
4 Thermo Fisher Scientific, San Jose, CA
5 Northeastern University, Boston, MA
United States
