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Reputable Mentor II
Reputable Mentor II
Mick Greer1, Vincent Gerbasi2, Joseph Greer3, Ryan Fellers3, David Horn4, Romain Huguet4, Susan Abbatiello5, Michael Belford4, Philip Compton2, Kenneth Durbin3, Neil Kelleher2, Richard LeDuc3, Scott Peterman4, Paul Thomas2

Purpose: Evaluate Thermo Scientific™ ProSightPD™ nodes for analysis of top-down FAIMS data. Methods: Samples were fractionated offline prior to LC separation coupled to a FAIMS enabled Orbitrap mass spectrometer. RAW files were imported directly into Thermo Scientific™ Proteome Discoverer™ 2.3 for analysis using ProSightPD™ 2.0 nodes. Results: ProSightPD software supports analysis of FAIMS separated top-down data collected at multiple CVs. Inclusion of FAIMS separation improved several top-down experimental metrics.

1 Thermo Fisher Scientific, Austin, TX, 2 Northwestern University, Evanston, IL 3 Proteinaceous Inc., Evanston, IL 4 Thermo Fisher Scientific, San Jose, CA 5 Northeastern University, Boston, MA
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Last update:
‎10-15-2021 11:23 AM
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